Sebastian Knust¹, Andy Sischka¹, Andre Spiering¹, and Dario Anselmetti¹ ¹: Experimental Biophysics & Applied Nanoscience, Faculty of Physics, Bielefeld University, 33615 Bielefeld, Germany Abstract For measuring the minute forces exerted on single molecules during controlled translocation through nanopores with sub-piconewton precision, we have developed a video-based axial force detection and analysis system for optical tweezers ...