For measuring the minute forces exerted on single molecules during controlled translocation through nanopores with sub-piconewton precision, we have developed a video-based axial force detection and analysis system for optical tweezers. Since our detection system is equipped with a standard and versatile CCD video camera with a limited bandwidth offering operation at moderate light illumination ...
Sebastian Knust¹, Andy Sischka¹, Andre Spiering¹, Christoph Pelargus¹, and Dario Anselmetti¹ ¹: Experimental Biophysics & Applied Nanoscience, Faculty of Physics, Bielefeld University, 33615 Bielefeld, Germany Abstract We developed and included video-based axial force analysis into our previously described optical tweezers setup [1]. By measuring the radius of a trapped microbead we achieve an overall force ...